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ACS Chem Biol. 2010 Jan 15;5(1):105-19. doi: 10.1021/cb900277e.

Phosphoproteomics for the masses.

Author information

1
Department of Chemistry,University of Wisconsin-Madison, Madison, WI 53706, USA.

Abstract

Protein phosphorylation serves as a primary mechanism of signal transduction in the cells of biological organisms. Technical advancements over the last several years in mass spectrometry now allow for the large-scale identification and quantitation of in vivo phosphorylation at unprecedented levels. These developments have occurred in the areas of sample preparation, instrumentation, quantitative methodology, and informatics so that today, 10 000-20 000 phosphorylation sites can be identified and quantified within a few weeks. With the rapid development and widespread availability of such data, its translation into biological insight and knowledge is a current obstacle. Here we present an overview of how this technology came to be and is currently applied, as well as future challenges for the field.

PMID:
20047291
PMCID:
PMC2810156
DOI:
10.1021/cb900277e
[Indexed for MEDLINE]
Free PMC Article

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