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Resuscitation. 2010 Feb;81(2):224-9. doi: 10.1016/j.resuscitation.2009.11.024. Epub 2009 Dec 31.

The state of systemic circulation, collapsed or preserved defines the need for hyperoxic or normoxic resuscitation in neonatal mice with hypoxia-ischemia.

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Department of Pediatrics, Division of Neonatology, Columbia University College of Physicians and Surgeons, 3959 Broadway, BHS1-115, Columbia University Medical Center, New York, NY 10032, USA.



The return of spontaneous circulation (ROSC) is a primary goal of resuscitation. For neonatal resuscitation the International Liaison Committee on Resuscitation (ILCOR) recommends oxygen concentrations ranging from 21% to 100%.


This study (a) compared the efficacy of resuscitation with room air (RA) or 100% O(2) in achieving ROSC in 46 neonatal mice with circulatory collapse induced by lethal hypoxia-ischemia (HI) and (b) determined whether re-oxygenation with RA or 100% O(2) alters the extent of HI cerebral injury in mice with preserved systemic circulation (n=31). We also compared changes in generation of reactive oxygen species (ROS) in cerebral mitochondria in response to re-oxygenation with RA or 100% O(2).


In HI-mice with collapsed circulation re-oxygenation with 100% O(2) versus RA resulted in significantly greater rate of ROSC. In HI-mice with preserved systemic circulation and regional (unilateral) cerebral ischemia the restoration of cerebral blood flow was significantly faster upon re-oxygenation with 100% O(2), than RA. However, no difference in the extent of brain injury was detected. Regardless of the mode of re-oxygenation, reperfusion in these mice was associated with markedly accelerated ROS production in brain mitochondria.


In murine HI associated with circulatory collapse the resuscitation limited to re-oxygenation with 100% O(2) is superior to the use of RA in achievement of the ROSC. However, in HI-mice with preserved systemic circulation hyperoxic re-oxygenation has no benefit over the normoxic brain recovery.

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