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Biotechniques. 2009 Dec;47(6):1041-3. doi: 10.2144/000113249.

Tunable fractionation of nucleic acids.

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Functional Genomics Technology Team, Omics Science Center (OSC), RIKEN Yokohama Institute, Kanagawa, Japan.


We developed a method for selective purification of DNA using the cationic detergent, cetyltrimethylammonium bromide (CTAB), accompanied with urea and controlled high-salt (NaCl) concentration. This method is effective for rapid separation of DNA fragments from artifacts such as PCR primer dimers or ligation adapters. The CTAB-associated purification completely removed the short PCR artifacts and primers, as well as enzymes and buffer, while recovering a sufficient quantity of amplicons for subsequent experiments such as preparation of libraries. This method could also be applied to the fractionation of nucleic acids generated by other types of reactions.

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