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Biotechniques. 1991 Jan;10(1):35-6, 38-9.

Producing single-stranded DNA probes with the Taq DNA polymerase: a high yield protocol.

Author information

1
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.

Erratum in

  • Biotechniques 1992 Mar;12(3):382.

Abstract

We report an efficient procedure to synthesize either single- or double-stranded probes labeled with biotin-11-dUTP, biotin-21-dUTP or digoxigenin-11-dUTP. To produce the single-stranded probe, only a single primer is utilized in a Taq polymerase amplification of 55 cycles. A cytomegalovirus probe is presented. This procedure allows easy production of nonradioactively labeled pure single-stranded probes of any desired length and specificity.

PMID:
2003918
[Indexed for MEDLINE]

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