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J Interferon Cytokine Res. 2010 May;30(5):311-20. doi: 10.1089/jir.2009.0082.

Differential transcriptional responses to interferon-alpha and interferon-gamma in primary human hepatocytes.

Author information

1
Department of Medicine, Stanford University School of Medicine , Stanford, California, USA.

Abstract

Interferon (IFN) plays a central role in the innate and adaptive antiviral immune responses. While IFN-alpha is currently approved for treating chronic hepatitis B and hepatitis C, in limited studies, IFN-gamma has not been shown to be effective for chronic hepatitis B or C. To identify the potential mechanism underlying the differential antiviral effects of IFN-alpha and IFN-gamma, we used cDNA microarray to profile the global transcriptional response to IFN-alpha and IFN-gamma in primary human hepatocytes, the target cell population of hepatitis viruses. Our results reveal distinct patterns of gene expression induced by these 2 cytokines. Overall, IFN-alpha induces more genes than IFN-gamma at the transcriptional level. Distinct sets of genes were induced by IFN-alpha and IFN-gamma with limited overlaps. IFN-alpha induces gene transcription at an early time point (6 h) but not at a later time point (18 h), while the effects of IFN-gamma are more prominent at 18 h than at 6 h, suggesting a delayed transcriptional response to IFN-gamma in the hepatocytes. These findings indicate differential actions of IFN-alpha and IFN-gamma in the context of therapeutic intervention for chronic viral infections in the liver.

PMID:
20038212
PMCID:
PMC2947462
DOI:
10.1089/jir.2009.0082
[Indexed for MEDLINE]
Free PMC Article

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