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Methods Mol Biol. 2010;612:429-46. doi: 10.1007/978-1-60761-362-6_29.

Activation of human NK cells by malaria-infected red blood cells.

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1
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK.

Abstract

This chapter describes a protocol to assess activation of human NK cells following in vitro stimulation with malaria-infected red blood cells. Activation is assessed by flow cytometry, staining for cell surface expression of CD69 and accumulation of intracellular IFN-gamma. Procedures are described for in vitro propagation and purification of Plasmodium falciparum parasites, separation of peripheral blood mononuclear cells from heparinised blood by density centrifugation, in vitro culture of PBMC and for staining and analysis of PBMC by flow cytometry. Some examples of typical FACS plots are shown.

PMID:
20033658
DOI:
10.1007/978-1-60761-362-6_29
[Indexed for MEDLINE]
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