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Anticancer Res. 2009 Nov;29(11):4331-6.

Establishment and characterization of a human gastrointestinal stromal tumour (GIST) xenograft in athymic nude mice.

Author information

1
Department of Nuclear Medicine, Rikshospitalet, Oslo University Hospital, 0027 Oslo, Norway. mona.elisabeth.revheim@radiumhospitalet.no

Abstract

BACKGROUND:

The majority of gastrointestinal stromal tumours (GISTs) contain oncogenic KIT (v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) or platelet-derived growth factor-alpha (PDGFRA) receptor tyrosine kinase (TK) mutations and are initially, but only temporarily sensitive to TK inhibitors. The aim of this study was to establish and characterize a human GIST xenograft that could be used for evaluating various molecularly targeted therapies.

MATERIALS AND METHODS:

GIST tissue from four patients was implanted under the skin of athymic nude mice. In one case a tumour line was established.

RESULTS:

The xenograft showed characteristic GIST morphology and exhibited the same mutation profile as that of the patient.

CONCLUSION:

A human GIST xenograft with mutation in KIT exons 11 and 17 has been established and maintained in nude mice for 3 years (13 passages). This model will enable further studies on mechanisms of resistance, combination therapies and allow testing of novel targeted therapies.

PMID:
20032375
[Indexed for MEDLINE]
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