Format

Send to

Choose Destination
See comment in PubMed Commons below
Int J Obes (Lond). 2010 Mar;34(3):487-99. doi: 10.1038/ijo.2009.263. Epub 2009 Dec 22.

Thyroid hormone responsive Spot 14 increases during differentiation of human adipocytes and its expression is down-regulated in obese subjects.

Author information

1
Service of Diabetes, Endocrinology and Nutrition, Institut d'Investigació Biomèdica de Girona, CIBEROBN (CB06/03/0010) and Instituto de Salud Carlos III, Girona, Spain.

Abstract

CONTEXT:

Very limited information is available regarding the function of human thyroid hormone responsive Spot 14 (human S14, hS14) in adipogenesis and human adiposity.

OBJECTIVE:

To evaluate hS14 levels during differentiation of human pre-adipocytes, in human fat depots and isolated fat cells.

DESIGN:

This was a cross-sectional study.

SUBJECTS:

A total of 161 omental (OM) and 87 subcutaneous (SC) adipose tissue samples obtained during elective surgical procedures from a population who varied widely in terms of obesity.

MEASUREMENTS:

hS14 gene expression and protein levels during adipogenesis were assessed by RT-PCR, western blot, and using an automated confocal imaging approach.

RESULTS:

hS14 gene expression levels were decreased in OM adipose tissue from overweight (-42.0%) and obese subjects (-56.5%) compared with lean subjects (P<0.05 and P<0.0001, respectively). hS14 mRNA (but not hS14-related) was inversely associated with obesity measures such as body mass index (P=0.001), percent fat mass (P=0.001), waist-to-hip ratio (P=0.020), and systolic blood pressure (P=0.031). hS14 gene expression and protein levels were up-regulated at the early stages of differentiation of human pre-adipocytes as well as for 3T3-L1 cells. That observation was most prominent in those individual cells exhibiting the more marked differentiation features. hS14 gene expression levels increased by approximately 45 000-fold in mature adipocytes. Increased hS14 levels were also found in stromal-vascular cells/pre-adipocytes (3.8-fold, P<0.05) and in adipose tissue samples (1.9-fold, P<0.0001) from SC compared with OM fat depots.

CONCLUSIONS:

These results suggest that hS14 is involved in human adipogenesis, but inversely related to obesity and OM fat accumulation.

PMID:
20029374
DOI:
10.1038/ijo.2009.263
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Support Center