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Q Rev Biophys. 2009 Aug;42(3):139-58. doi: 10.1017/S0033583509990059.

Single-particle reconstruction of biological macromolecules in electron microscopy--30 years.

Author information

1
The Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA. jf2192@columbia.edu

Abstract

This essay gives the autho's personal account on the development of concepts underlying single-particle reconstruction, a technique in electron microscopy of macromolecular assemblies with a remarkable record of achievements as of late. The ribosome proved to be an ideal testing ground for the development of specimen preparation methods, cryo-EM techniques, and algorithms, with discoveries along the way as a rich reward. Increasingly, cryo-EM and single-particle reconstruction, in combination with classification techniques, is revealing dynamic information on functional molecular machines uninhibited by molecular contacts.

PMID:
20025794
PMCID:
PMC2844734
DOI:
10.1017/S0033583509990059
[Indexed for MEDLINE]
Free PMC Article

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