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Anal Chem. 2010 Jan 15;82(2):491-7. doi: 10.1021/ac902139w.

Nonlinear optical imaging of integral membrane protein crystals in lipidic mesophases.

Author information

1
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.

Abstract

Second-order nonlinear optical imaging of chiral crystals (SONICC) is explored for selective detection of integral membrane protein crystals grown in opaque and turbid environments. High turbidity is a hallmark of membrane protein crystallization due to the extensive use of detergent and/or lipids that often form various mesophases. Detection of crystals in such media by conventional optical methods (e.g., intrinsic UV fluorescence, birefringence, bright-field image analysis, etc.) is often complicated by optical scattering and by the small sizes of the crystals that routinely form. SONICC is shown to be well-suited for this application, by nature of its compatibility with imaging in scattering media and its high selectivity for protein crystals. Bright second harmonic generation (SHG) (up to 18 million counts/s) was observed from even relatively small crystals (5 mum) with a minimal background due to the surrounding lipid mesophase ( approximately 1 thousand counts/s). The low background nature of the resulting protein crystal images permitted the use of a relatively simple, particle counting analysis for preliminary scoring. Comparisons between a particle counting analysis of SONICC images and protocols based on the human expert analysis of conventional bright-field and birefringence images were performed.

PMID:
20025250
PMCID:
PMC2825164
DOI:
10.1021/ac902139w
[Indexed for MEDLINE]
Free PMC Article

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