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Org Biomol Chem. 2009 Dec 21;7(24):5055-8. doi: 10.1039/b917119n. Epub 2009 Oct 26.

Clickable fluorescent dyes for multimodal bioorthogonal imaging.

Author information

1
The Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, NY 10065, USA.

Abstract

Bioorthogonal ligation with functionalized fluorescent dyes enables visualization of nuclei acids, proteins and metabolites in biological systems. Bright and modular azide- and alkyne-functionalized dyes are therefore needed to expand the fluorescence imaging capabilities of bioorthogonal ligation methods. We describe the concise synthesis of clickable fluorescent dyes based on 2-dicyanomethylene-3-cyano-2,5-dihydrofuran fluorophores and demonstrate their utility for multicolor imaging of azide- and alkyne-modified proteins as well as FRET studies.

PMID:
20024096
DOI:
10.1039/b917119n
[Indexed for MEDLINE]

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