Microbacterium lindanitolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil

Int J Syst Evol Microbiol. 2010 Nov;60(Pt 11):2634-2638. doi: 10.1099/ijs.0.017699-0. Epub 2009 Dec 18.

Abstract

A Gram-stain-positive, aerobic, rod-shaped, yellow actinobacterium, designated MNA2(T), was isolated from a hexachlorocyclohexane-contaminated soil in North India. Strain MNA2(T) showed 95 % 16S rRNA gene sequence similarity with Microbacterium halotolerans YIM 70130(T). Phylogenetic analysis of 16S rRNA gene sequences showed that strain MNA2(T) belonged to a clade represented by the genus Microbacterium of the family Microbacteriaceae. Strain MNA2(T) contained anteiso-C(15 : 0), anteiso-C(17 : 0), iso-C(16 : 0) and iso-C(15 : 0) as the predominant fatty acids and diphosphatidylglycerol, phosphatidylglycerol and two unknown polar lipids. The menaquinones were MK-12, MK-11, MK-13 and MK-10, in an approximate molar ratio of 45 : 40 : 13 : 3, respectively. The DNA G+C content was 65.3 mol%. The peptidoglycan was of the B type of cross-linkage with ornithine as the diagnostic diamino acid. The results of the phylogenetic, phenotypic and chemotaxonomic analysis indicate that strain MNA2(T) belongs to a previously unrecognized species of the genus Microbacterium, for which the name Microbacterium lindanitolerans sp. nov. is proposed. The type strain is MNA2(T) (=DSM 22422(T) =CCM 7585(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / classification*
  • Actinomycetales / genetics
  • Actinomycetales / isolation & purification*
  • Actinomycetales / metabolism
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / metabolism
  • Hexachlorocyclohexane / metabolism*
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Soil Microbiology*
  • Soil Pollutants / metabolism*

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Soil Pollutants
  • Hexachlorocyclohexane

Associated data

  • GENBANK/EU873539