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J Biol Chem. 1991 Mar 15;266(8):5162-8.

Saccharomyces cerevisiae transcription factors IIIB and IIIC bend the DNA of a tRNA(Gln) gene.

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  • 1Department of Biology, University of California, San Diego, La Jolla 92093.


The ability of Saccharomyces cerevisiae transcription factors IIIC and IIIB to bend DNA at their binding sites in a tRNA(Gln) gene has been analyzed by two methods that are based on the anomalous electrophoretic mobility of bent DNA in a tight gel network. Both transcription factors bend DNA, TFIIIB more than TFIIIC. The effective centers of the transcription factor IIIB- and IIIC-induced bends are not in helical phase.

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