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FEBS Lett. 1991 Feb 25;279(2):210-5.

Cloning and sequencing of Lol pI, the major allergenic protein of rye-grass pollen.

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ImmuLogic Pharmaceutical Corporation, Cambridge, MA 02139.


We have isolated a full length cDNA clone encoding the major glycoprotein allergen Lol pI. The clone was selected using a combination of immunological screening of a cDNA expression library and PCR amplification of Lol pI-specific transcripts. Lol pI expressed in bacteria as a fusion protein shows recognition by specific IgE antibodies present in sera of grass pollen-allergic subjects. Northern analysis has shown that the Lol pI transcripts are expressed only in pollen of rye-grass. Molecular cloning of Lol pI provides a molecular genetic approach to study the structure-function relationship of allergens.

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