Format

Send to

Choose Destination
FEBS Lett. 1991 Feb 25;279(2):210-5.

Cloning and sequencing of Lol pI, the major allergenic protein of rye-grass pollen.

Author information

1
ImmuLogic Pharmaceutical Corporation, Cambridge, MA 02139.

Abstract

We have isolated a full length cDNA clone encoding the major glycoprotein allergen Lol pI. The clone was selected using a combination of immunological screening of a cDNA expression library and PCR amplification of Lol pI-specific transcripts. Lol pI expressed in bacteria as a fusion protein shows recognition by specific IgE antibodies present in sera of grass pollen-allergic subjects. Northern analysis has shown that the Lol pI transcripts are expressed only in pollen of rye-grass. Molecular cloning of Lol pI provides a molecular genetic approach to study the structure-function relationship of allergens.

PMID:
2001733
DOI:
10.1016/0014-5793(91)80151-r
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center