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Rev Bras Cir Cardiovasc. 2009 Jul-Sep;24(3):312-7.

Expression of apoptosis in human saphenous vein grafts in restoration of blood flow through coronary bypass surgery.

Author information

1
FMRP - USP. tirapeli@fmrp.usp.br

Abstract

OBJECTIVE:

To investigate the possible role of apoptosis on brief distensions of human saphenous veins at different pressures.

METHODS:

Fresh isolated grafts of human saphenous vein were assigned as control or distended (D) for fifteen seconds at 100, 200 and 300 mmHg. The degree of apoptotic caspases 3, 8, 9 and anti-apoptotic protein Bcl-2 expression were assessed by immunohistochemistry.

RESULTS:

Fresh isolated segments of distended human saphenous veins presented similar apoptotic protein expression when compared with control veins. However, the Bcl-2 expression was significantly higher in the 300 mmHg distended segments compared with the control vein.

CONCLUSION:

These findings show that intact segments of human saphenous veins submitted to distensions at different pressures have similar apoptotic proteins expression when compared with non-distended control veins. Therefore, brief distensions commonly performed during surgical harvesting do not trigger apoptosis, and probably are not involved on the physiopathological mechanisms that lead to graft failure.

PMID:
20011876
DOI:
10.1590/s0102-76382009000400009
[Indexed for MEDLINE]

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