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Nat Methods. 2010 Jan;7(1):56-9. doi: 10.1038/nmeth.1410. Epub 2009 Dec 13.

Single-gene transgenic mouse strains for reprogramming adult somatic cells.

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1
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.

Abstract

We report transgenic mouse models in which three or four reprogramming factors are expressed from a single genomic locus using a drug-inducible transgene. Multiple somatic cell types can be directly reprogrammed to generate induced pluripotent stem cells (iPSCs) by culture in doxycycline. Because reprogramming factors are carried on a single polycistronic construct, the mice can be easily maintained, and the transgene can be easily transferred into other genetic backgrounds.

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PMID:
20010831
PMCID:
PMC3048025
DOI:
10.1038/nmeth.1410
[Indexed for MEDLINE]
Free PMC Article
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