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EMBO Rep. 2010 Jan;11(1):52-8. doi: 10.1038/embor.2009.254. Epub 2009 Dec 4.

Intergenic transcripts originating from a subclass of ribosomal DNA repeats silence ribosomal RNA genes in trans.

Author information

1
Division of Molecular Biology of the Cell II, German Cancer Research Center, DKFZ-ZMBH-Alliance, INF 581, 69120 Heidelberg, Germany.

Abstract

Epigenetic silencing of a fraction of ribosomal DNA (rDNA) requires association of the nucleolar chromatin-remodelling complex NoRC to 150-250 nucleotide RNAs (pRNA) that originate from an RNA polymerase I promoter located in the intergenic spacer separating rDNA repeats. Here, we show that NoRC-associated pRNA is transcribed from a sub-fraction of hypomethylated rRNA genes during mid S phase, acting in trans to inherit DNA methylation and transcriptional repression of late-replicating silent rDNA copies. The results reveal variability between individual rDNA clusters with distinct functional consequences.

PMID:
20010804
PMCID:
PMC2816622
DOI:
10.1038/embor.2009.254
[Indexed for MEDLINE]
Free PMC Article

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