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Science. 2010 Jan 22;327(5964):466-9. doi: 10.1126/science.1179663. Epub 2009 Dec 10.

Tuberculous granuloma induction via interaction of a bacterial secreted protein with host epithelium.

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1
Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, WA 98155, USA.

Abstract

Granulomas, organized aggregates of immune cells, are a hallmark of tuberculosis and have traditionally been thought to restrict mycobacterial growth. However, analysis of Mycobacterium marinum in zebrafish has shown that the early granuloma facilitates mycobacterial growth; uninfected macrophages are recruited to the granuloma where they are productively infected by M. marinum. Here, we identified the molecular mechanism by which mycobacteria induce granulomas: The bacterial secreted protein 6-kD early secreted antigenic target (ESAT-6), which has long been implicated in virulence, induced matrix metalloproteinase-9 (MMP9) in epithelial cells neighboring infected macrophages. MMP9 enhanced recruitment of macrophages, which contributed to nascent granuloma maturation and bacterial growth. Disruption of MMP9 function attenuated granuloma formation and bacterial growth. Thus, interception of epithelial MMP9 production could hold promise as a host-targeting tuberculosis therapy.

PMID:
20007864
PMCID:
PMC3125975
DOI:
10.1126/science.1179663
[Indexed for MEDLINE]
Free PMC Article

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