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Biomaterials. 2010 Mar;31(8):2229-35. doi: 10.1016/j.biomaterials.2009.11.098. Epub 2009 Dec 11.

Control of cell attachment through polyDNA hybridization.

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1
Radioisotope Research Center, Kyoto University, Yoshida-Konoe-Cho, Sakyo-ku, Kyoto, 606-8501, Japan.

Abstract

Cell-cell interactions play vital roles in embryo development and in homeostasis maintenance. Such interactions must be stringently controlled for cell-based tissue engineering and regenerative medicine therapies, and methods for studying and controlling cell-cell interactions are being developed using both biomedical and engineering approaches. In this study, we prepared amphiphilic PEG-lipid polymers that were attached to polyDNA with specific sequences. Incubation of cells with the polyDNA-PEG-lipid conjugate transferred some of the polyDNA to the cells' surfaces. Similarly, polyDNA-PEG-lipid conjugate using polyDNA with a complementary sequence was introduced to the surfaces of other cells or to a substrate surface. Cell-cell or cell-substrate attachments were subsequently mediated via hybridization between the two complementary polyDNAs and monitored using fluorescence microscopy.

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