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Ocul Immunol Inflamm. 2009 Nov-Dec;17(6):380-9. doi: 10.3109/09273940903118642.

The immune privileged retina mediates an alternative activation of J774A.1 cells.

Author information

1
Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA 02116, USA.

Abstract

PURPOSE:

We have previously found that retinal pigment epithelial (RPE) cells suppressed endotoxin-stimulated macrophages; moreover, it induced expression of anti-inflammatory cytokines. We further assessed the possibility that the RPE is alternatively activating macrophages.

METHODS:

J774A.1 cells were stimulated with endotoxin and treated with the conditioned media (CM) of RPE, or neuroretinal eyecups from healthy mouse eyes. The supernatant was assayed for IL-1 beta, TNF-alpha, IL-6, IL-12(p70), and IL-10, and for nitric-oxide generation. The RPE conditioned media (RPE CM) was absorbed of known soluble factors to identify the factor that augments nitric-oxide generation.

RESULTS:

We found the RPE CM suppressed all cytokine production except IL-10, and augmented nitric-oxide generation. The augmented nitric-oxide levels were mediated by RPE derived alpha-melanocyte stimulating hormone (alpha-MSH).

CONCLUSIONS:

Healthy RPE not only suppresses inflammatory activity, it promotes an alternative activation of macrophages that can further promote immune privilege.

PMID:
20001256
PMCID:
PMC4698149
DOI:
10.3109/09273940903118642
[Indexed for MEDLINE]
Free PMC Article

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