Protein-resistant polymer coatings based on surface-adsorbed poly(aminoethyl methacrylate)/poly(ethylene glycol) copolymers

Biomacromolecules. 2010 Jan 11;11(1):233-7. doi: 10.1021/bm901082y.

Abstract

We report on the protein-resistant properties of glass substrates coated with novel copolymers of 2-aminoethyl methacrylate hydrochloride and poly(ethylene glycol) methyl ether methacrylate (AEM-PEG). In comparison to currently available protein-blocking polymer systems, such as poly-l-lysine-poly(ethylene glycol), silane-based poly(ethylene glycol), and poly(ethylene glycol) brushes prepared by surface-initiated polymerization, the proposed AEM-PEG offers the combined advantages of low cost, simplicity of use, and applicability in aqueous solutions. We demonstrate the capability of AEM-PEG to block the surface binding of globular proteins (tubulin), their assemblies (microtubules), and functional motor proteins (kinesin-1). Moreover, we demonstrate the applicability of AEM-PEG for surface patterning of proteins in microfluidic devices.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Kinesins / chemistry*
  • Kinesins / metabolism
  • Methacrylates / chemistry*
  • Microfluidic Analytical Techniques
  • Microtubules / chemistry*
  • Microtubules / metabolism
  • Polyethylene Glycols / chemistry*
  • Polymers / chemistry*
  • Polymers / metabolism
  • Silanes / chemistry*
  • Tubulin / chemistry*
  • Tubulin / metabolism

Substances

  • Methacrylates
  • Polymers
  • Silanes
  • Tubulin
  • poly(ethylene glycol)silane
  • Polyethylene Glycols
  • 2-aminoethylmethacrylate
  • Kinesins