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Nat Methods. 2010 Jan;7(1):50-2. doi: 10.1038/nmeth.1406. Epub 2009 Dec 6.

Floxin, a resource for genetically engineering mouse ESCs.

Author information

1
Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.

Abstract

We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.

PMID:
19966808
PMCID:
PMC2895430
DOI:
10.1038/nmeth.1406
[Indexed for MEDLINE]
Free PMC Article

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