Format

Send to

Choose Destination
See comment in PubMed Commons below
Biosci Biotechnol Biochem. 2009 Dec;73(12):2783-6. Epub 2009 Dec 7.

Minimum length of homology arms required for effective Red/ET recombination.

Author information

1
Graduate School of Innovative Life Science, University of Toyama, Japan.

Abstract

The original protocol of Red/ET recombination requires 50-bp sequence homology with target vector on both sides of the DNA fragment. To make it more cost effective, we investigated to determine the minimal length of homology required for the system to work. We found that a homology of 9-bp was sufficient to achieve homologous recombination with more than 50% efficiency.

PMID:
19966478
DOI:
10.1271/bbb.90584
[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Taylor & Francis Icon for J-STAGE, Japan Science and Technology Information Aggregator, Electronic
    Loading ...
    Support Center