Diagnostic assay for Rickettsia japonica

Nozomu Hanaoka; Minenosuke Matsutani; Hiroki Kawabata; Seigo Yamamoto; Hiromi Fujita; Akiko Sakata; Yoshinao Azuma; Motohiko Ogawa; Ai Takano; Haruo Watanabe; Toshio Kishimoto; Mutsunori Shirai; Ichiro Kurane; Shuji Ando

doi:10.3201/eid1512.090252

Diagnostic assay for Rickettsia japonica

Emerg Infect Dis. 2009 Dec;15(12):1994-7. doi: 10.3201/eid1512.090252.

Authors

Nozomu Hanaoka¹, Minenosuke Matsutani, Hiroki Kawabata, Seigo Yamamoto, Hiromi Fujita, Akiko Sakata, Yoshinao Azuma, Motohiko Ogawa, Ai Takano, Haruo Watanabe, Toshio Kishimoto, Mutsunori Shirai, Ichiro Kurane, Shuji Ando

Affiliation

¹ National Institute of Infectious Diseases, Tokyo 162-8640, Japan.

Abstract

We developed a specific and rapid detection system for Rickettsia japonica and R. heilongjiangensis, the causative agents of spotted fever, using a TaqMan minor groove binder probe for a particular open reading frame (ORF) identified by the R. japonica genome project. The target ORF was present only in R. japonica-related strains.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Aged, 80 and over
Base Sequence
Female
Humans
Male
Middle Aged
Molecular Sequence Data
Open Reading Frames
Polymerase Chain Reaction
Rickettsia / genetics
Rickettsia / isolation & purification*
Rickettsia Infections / diagnosis