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Am J Nephrol. 2010;31(2):160-4. doi: 10.1159/000264634. Epub 2009 Dec 3.

An oral sorbent, AST-120, increases Klotho expression and inhibits cell senescence in the kidney of uremic rats.

Author information

1
Clinical Preventive Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan.

Abstract

BACKGROUND/AIM:

Klotho, an anti-aging gene, is primarily expressed in the kidney, and its renal expression is decreased in chronic renal failure (CRF). We determined if administration of an oral sorbent, AST-120, increases the expression of Klotho, and inhibits cell senescence in the kidney of CRF rats.

METHODS:

CRF rats were produced by 4/5-nephrectomy. AST-120 was administered to the CRF rats at a dose of 4 g/kg with powder chow for 16 weeks, whereas powder chow alone was administered to control CRF rats. The expression of Klotho and cell senescence (senescence-associated beta-galactosidase: SA-beta-gal) was detected by immunohistochemistry.

RESULTS:

The expression of Klotho was significantly decreased in the kidney of CRF rats as compared with normal rats. AST-120-treated CRF rats showed significantly increased renal expression of Klotho as compared with CRF rats. The expression of SA-beta-gal was significantly increased in the kidney of CRF rats as compared with normal rats. AST-120-treated CRF rats showed significantly decreased expression of SA-beta-gal in the kidney as compared with CRF rats. AST-120 significantly decreased serum and urine levels of indoxyl sulfate.

CONCLUSIONS:

AST-120 increased Klotho expression, and inhibited cell senescence in the kidney of CRF rats, probably by alleviating indoxyl sulfate overload on the kidney.

PMID:
19955715
DOI:
10.1159/000264634
[Indexed for MEDLINE]

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