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Biomaterials. 2010 Mar;31(7):1699-706. doi: 10.1016/j.biomaterials.2009.11.032. Epub 2009 Dec 1.

Poly(L-lysine) nanostructured particles for gene delivery and hormone stimulation.

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1
Institut National de la Santé et de la Recherche Médicale, INSERM, Unité 977, Faculté de Médecine, 11 Rue Humann, 67085 Strasbourg Cedex, France.

Abstract

In this work, we designed replica particles based on poly (L-lysine) (PLL) polymers crosslinked via a homobifunctional linker to support coadsorption of a plasmid DNA and a peptide hormone for concurrent transfection and induction of a cellular function. PLL replica particles (PLL(RP)) were prepared by infiltrating polymer into mesoporous silica (MS) particles, crosslinking the adsorbed chains by using a homobifunctional crosslinker and finally removing the template particles. Moreover, we verified their cytotoxicity. Furthermore, based on this PLL(RP) gene delivery system, we simultaneously evaluated the melanin stimulation and gene expression in these cells by fluorescence microscopy. To further understand the bi-functionality, we labeled the SPT7pTL and PGA-alpha-MSH with YOYO-1 and Rhodamine, respectively, to follow its intracellular pathway by confocal microscopy. Our data suggests that the PLL(RP) is a promising vector for gene therapy and hormone stimulation.

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