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Opt Lett. 2009 Dec 1;34(23):3668-70. doi: 10.1364/OL.34.003668.

Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells.

Author information

1
George R. Harrison Spectroscopy Laboratory, MIT, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

Abstract

We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual human red blood cells. The end point of this study is noninvasive monitoring of physiological states of intact living cells.

PMID:
19953156
PMCID:
PMC2848941
DOI:
10.1364/OL.34.003668
[Indexed for MEDLINE]
Free PMC Article

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