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Exp Cell Res. 1991 Mar;193(1):151-4.

Identification of a brain-specific 27/26-kDa extracellular protein with the monoclonal antibody to differentiated PC12h pheochromocytoma cells.

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1
Department of Biology, Ochanomizu University, Tokyo, Japan.

Abstract

We have searched for brain-specific extracellular molecules using a library of monoclonal antibodies against surface antigens of differentiated PC12h cells. One of the monoclonal antibodies, PCH42-14, recognized a 27/26-kDa protein of 10-week-old rat brain on immunoblotting. PCH42-14 antigen was detected only in brain, especially in cerebrum, olfactory bulb, mesencephalon, hippocampus, medulla oblongata, and spinal cord. On hippocampal neuron culture, PCH42-14 antigen existed extracellularly along with the neuronal extensions.

PMID:
1995290
[Indexed for MEDLINE]
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