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Quantitation of fungal DNA contamination in commercial zymolyase and lyticase used in the preparation of fungi.

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1
Teikyo University Institute of Medical Mycology.

Abstract

Small amounts of contaminants may lead to false-positive results in sensitive polymerase chain reaction (PCR) detection systems. To analyze contaminants and understand the usability of beta-glucanases in fungal preparations, we estimated the ribosomal DNA (rDNA) contamination in Zymolyase-100T and Lyticase by quantitative PCR. The amount of rDNA contamination determined by real-time PCR was 9210 copies/unit for Zymolyase-100T and 0.0323 copies/unit for Lyticase. The observations regarding these enzyme products indicate that careful consideration of contaminating DNA included in the reagents used for molecular diagnostics is necessary.

PMID:
19942798
DOI:
10.3314/jjmm.50.259
[Indexed for MEDLINE]
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