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Nat Rev Mol Cell Biol. 2009 Dec;10(12):866-76. doi: 10.1038/nrm2805.

Exploring protein fitness landscapes by directed evolution.

Author information

1
Division of Chemistry and Chemical Engineering, 21041, California Institute of Technology, Pasadena, California 91125, USA. promero@caltech.edu

Abstract

Directed evolution circumvents our profound ignorance of how a protein's sequence encodes its function by using iterative rounds of random mutation and artificial selection to discover new and useful proteins. Proteins can be tuned to adapt to new functions or environments by simple adaptive walks involving small numbers of mutations. Directed evolution studies have shown how rapidly some proteins can evolve under strong selection pressures and, because the entire 'fossil record' of evolutionary intermediates is available for detailed study, they have provided new insight into the relationship between sequence and function. Directed evolution has also shown how mutations that are functionally neutral can set the stage for further adaptation.

PMID:
19935669
PMCID:
PMC2997618
DOI:
10.1038/nrm2805
[Indexed for MEDLINE]
Free PMC Article

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