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FEBS Lett. 1991 Jan 14;278(1):98-102.

Reduction of biological activity of murine recombinant interleukin-1 beta by selective deamidation at asparagine-149.

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Central Research Division, Pfizer Inc., Groton, CT 06340.


A biologically active preparation of murine recombinant interleukin-1 beta (mIL-1 beta) from Escherichia coli cell lysates contained tow forms of mIL-1 beta with pI 8.7 and pI 8.1, respectively. Treatment with 0.1 M Tris, pH 8.5, at 37 degrees C for 35 h converted the pI 8.7 form to the pI 8.1 form by the selective deamidation of an asparagine residue (Asn149) in the mIL-1 beta molecule. Deamidated mIL-1 beta had 3- to 5-fold lower co-mitogenic activity and receptor affinity than the unmodified form.

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