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Biochemistry. 2009 Dec 22;48(50):11834-6. doi: 10.1021/bi901756n.

A peptide tag system for facile purification and single-molecule immobilization.

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1
Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois 60637, USA.

Abstract

A peptide fusion tag and accompanying recombinant capture reagents have been developed on the basis of the peptide-PDZ domain interaction and affinity clamps, a new class of affinity reagent. This system allows for single-step purification under mild conditions and stable capture of a tagged protein. The subnanomolar affinity, high force resistance (>30 pN), small size ( approximately 25 kDa, approximately one-sixth of the size of IgG), and monomeric nature of the affinity clamp are all superior features for many applications, in particular single-molecule measurements.

PMID:
19928925
PMCID:
PMC2795123
DOI:
10.1021/bi901756n
[Indexed for MEDLINE]
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