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Biochemistry. 2009 Dec 22;48(50):11837-9. doi: 10.1021/bi901839d.

Nonspecificity of binding of gamma-secretase modulators to the amyloid precursor protein.

Author information

1
Department of Biochemistry and Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37232-8725, USA.

Abstract

Evidence that certain gamma-secretase modulators (GSMs) target the 99-residue C-terminal domain (C99) of the amyloid precursor protein, a substrate of gamma-secretase, but not the protease complex itself has been presented [Kukar, T. L., et al. (2008) Nature 453, 925-929]. Here, NMR results demonstrate a lack of specific binding of these GSMs to monodisperse C99 in LMPG micelles. In addition, results indicate that C99 was likely to have been aggregated in some of the key experiments of the previous work and that binding of GSMs to these C99 aggregates is also of a nonspecific nature.

PMID:
19928774
PMCID:
PMC2794937
DOI:
10.1021/bi901839d
[Indexed for MEDLINE]
Free PMC Article

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