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FEBS Lett. 2010 Jan 4;584(1):153-8. doi: 10.1016/j.febslet.2009.11.042.

Overexpression of the single-stranded DNA-binding protein (SSB) stabilises CAG*CTG triplet repeats in an orientation dependent manner.

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1
Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.

Abstract

The stability and deletion-size-distribution profiles of leading strand (CAG)(75) and (CTG)(137) trinucleotide repeat arrays inserted in the Escherichia coli chromosome were investigated upon overexpression of the single-stranded DNA-binding protein (SSB) and in mutant strains deficient for the SbcCD (Rad51/Mre11) nuclease. SSB overexpression increases the stability of the (CAG)(75) repeat array and leads to a loss of the bias towards large deletions for the same array. Furthermore, the absence of SbcCD leads to a reduction in the number of large deletions in strains containing the (CTG)(137) repeat array.

PMID:
19925793
DOI:
10.1016/j.febslet.2009.11.042
[Indexed for MEDLINE]
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