Format

Send to

Choose Destination
PLoS One. 2009 Nov 16;4(11):e7844. doi: 10.1371/journal.pone.0007844.

Transcriptome profiling of human pre-implantation development.

Author information

1
Department of Bioscience and Nutrition, Karolinska Institutet, Stockholm, Sweden.

Abstract

BACKGROUND:

Preimplantation development is a crucial step in early human development. However, the molecular basis of human preimplantation development is not well known.

METHODOLOGY:

By applying microarray on 397 human oocytes and embryos at six developmental stages, we studied the transcription dynamics during human preimplantation development.

PRINCIPAL FINDINGS:

We found that the preimplantation development consisted of two main transitions: from metaphase-II oocyte to 4-cell embryo where mainly the maternal genes were expressed, and from 8-cell embryo to blastocyst with down-regulation of the maternal genes and up-regulation of embryonic genes. Human preimplantation development proved relatively autonomous. Genes predominantly expressed in oocytes and embryos are well conserved during evolution.

SIGNIFICANCE:

Our database and findings provide fundamental resources for understanding

PMID:
19924284
PMCID:
PMC2773928
DOI:
10.1371/journal.pone.0007844
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center