A side-by-side comparison was made between a conventional vial system and a novel bottle system for cultivating flies and testing the effect of chemical exposure on the lifespan of the flies. While the two cultivation systems yielded very similar results for the effect of DEHP (di[2-ethylhexyl] phthalate) on reducing the lifespan of fruit fly Drosophila melanogaster, the new bottle system has many advantages over the conventional vial system. The bottle system allowed long-term cultivation of flies in the same bottle and thus eliminated the need for transferring of flies between vials. Foods/nutrients were provided as fresh moisture medium coated on a glass slide vertically hanged in the center of the bottle. Fly discharges and dead flies were collected onto a draw horizontally inserted into the bottom of the bottle. These features have resulted in great convenience for cultivating flies and reduced labor and media cost. The effective separation of food from discharge may allow accurate mass balance measurement and thus yield more definitive observations for understanding the actual role of calorie restriction (CR) or dietary-restriction (DR) in fly metabolism and longevity.
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