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Biochemistry. 2009 Dec 15;48(49):11609-11. doi: 10.1021/bi901630j.

Contrasting synergistic anion effects in vanadium(V) binding to nicatransferrin versus human serum transferrin.

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1
Department of Chemistry, Yale University, New Haven, Connecticut 06520-8107, USA.

Abstract

Some ascidians sequester vanadium and other metal ions that are bound and transported in higher organisms by transferrin. The ascidian Ciona intestinalis has a monolobal transferrin (nicatransferrin) in its plasma. The binding of vanadium(V) to nicatransferrin was investigated by using isothermal titration calorimetry and UV-vis spectroscopy, in the presence and absence of NaHCO(3), and was compared with human serum transferrin. Nicatransferrin and serum transferrin bind V(V) with similar strengths [K = (2.0 +/- 0.6) x 10(5) M(-1) for nicatransferrin]; however, nicatransferrin requires a synergistic anion for V(V) binding, whereas serum transferrin does not. Spectroscopy supports a different kind of coordination site.

PMID:
19921860
DOI:
10.1021/bi901630j
[Indexed for MEDLINE]
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