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Inflamm Res. 2010 Jun;59(6):429-36. doi: 10.1007/s00011-009-0114-7. Epub 2009 Nov 19.

Extramedullary hematopoiesis is dysregulated in histamine-free histidine decarboxylase knockout (HDC-/-) mice.

Author information

1
Department of Genetics, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, Hungary.

Abstract

OBJECTIVE AND DESIGN:

In this study we investigated the role of histamine on the extramedullary hematopoiesis.

METHODS:

Male histidine decarboxylase knockout (HDC(-/-)) mice and wild-type mice were used (n = 5/group). Groups of mice received sublethal total-body gamma irradiation at a single dose of 4 Gy. Spleen cells were studied at different time points post-irradiation by flow cytometry, colony forming unit (CFU) assay, and real-time PCR. For statistical analysis Student's t test, ANOVA, and Holm-Sidak post-hoc test were used.

RESULTS:

By day 14 after irradiation, spleen cell counts increased almost eightfold in wild-type and not even fourfold in HDC(-/-) mice (P < 0.01). The proliferative capacity and interleukin-3 signaling of stem cells were impaired in HDC(-/-) mice. STAT5 mRNA expression was decreased in granulocyte-myeloid colonies by 72.9 +/- 8.6% (P < 0.001), compared to the wild-type.

CONCLUSIONS:

The absence of histamine adversely affects splenic hematopoiesis via direct and indirect mechanisms.

PMID:
19921486
DOI:
10.1007/s00011-009-0114-7
[Indexed for MEDLINE]

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