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J Infect Dis. 2009 Dec 15;200(12):1916-20. doi: 10.1086/648473.

Enhanced expression of dltABCD is associated with the development of daptomycin nonsusceptibility in a clinical endocarditis isolate of Staphylococcus aureus.

Author information

1
Los Angeles Biomedical Research Institute at Harbor-UCLA, Torrance, CA 90502, USA. sjyang@labiomed.org

Abstract

Using isogenic clinical bloodstream Staphylococcus aureus strains from a patient with relapsing endocarditis, we investigated the transcriptional profiles of the mprF and dlt genes in the context of cell-surface charge and daptomycin nonsusceptibility. As in prior studies, a point mutation within mprF was observed in the daptomycin-nonsusceptible strain. However, neither the transcriptional profile of mprF nor the membrane phospholipid analyses were compatible with the anticipated mprF gain-in-function phenotype. In contrast, we demonstrated enhanced dlt expression coincident with increased positive surface charge and reduced daptomycin binding.

PMID:
19919306
PMCID:
PMC2779839
DOI:
10.1086/648473
[Indexed for MEDLINE]
Free PMC Article

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