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J Photochem Photobiol B. 2010 Jan 21;98(1):7-11. doi: 10.1016/j.jphotobiol.2009.10.001. Epub 2009 Nov 13.

The effects of formins on the conformation of subdomain 1 in actin filaments.

Author information

1
University of Pécs, Faculty of Medicine, Department of Biophysics, Pécs, Szigeti str. 12, H-7624, Hungary.

Abstract

In this study we investigated the effects of formins on the conformation of actin filaments by using the method of fluorescence quenching. Actin was labelled with IAEDANS at Cys(374) and the quencher was acrylamide. The results showed that formin binding induced structural changes in the subdomain 1 of actin protomers which were reflected by greater quenching constants (K(SV)). Simultaneously the fraction of the fluorophore population accessible for the quencher (alpha) decreased. These observations suggest that the conformational distribution characteristic for the actin protomers became broader after the binding of formins, for which the structural framework was provided by a more flexible protein matrix in the microenvironment of the label. The effects of formins depended on the formin:actin molar ratio, and also on the ionic strength of the medium. These observations are in agreement with previous results and underline the importance of the intramolecular conformational changes induced by formins in the structure of actin filaments.

PMID:
19914084
PMCID:
PMC2865993
DOI:
10.1016/j.jphotobiol.2009.10.001
[Indexed for MEDLINE]
Free PMC Article

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