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Methods Mol Biol. 2010;585:93-105. doi: 10.1007/978-1-60761-380-0_8.

Expression and analysis of exogenous proteins in epidermal cells.

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1
Department of Physiology & Pharmacology, Child Health Research Institute and Lawson Health Research Institute, University of Western Ontario, London, ON, Canada.

Abstract

In this chapter we review protocols for transient transfection of primary keratinocytes. The ability to transfect primary epidermal cells regardless of their differentiation status allows the biochemical and molecular characterization of multiple proteins. We review methods to analyze exogenous protein abundance in transfected keratinocytes by immunoblot and immunoprecipitation. We also present protocols to determine the subcellular distribution of these proteins by indirect immunofluorescence microscopy approaches.

PMID:
19907999
DOI:
10.1007/978-1-60761-380-0_8
[Indexed for MEDLINE]
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