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PLoS One. 2009 Nov 10;4(11):e7637. doi: 10.1371/journal.pone.0007637.

Visualization and identification of IL-7 producing cells in reporter mice.

Author information

1
Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, NIH, Frederick, MD, USA.

Abstract

Interleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in the Il7 locus. The construct lacked a signal peptide and ECFP (enhanced cyan fluorescent protein) accumulated inside IL-7-producing stromal cells in thoracic thymus, cervical thymus and bone marrow. In thymus, an extensive reticular network of IL-7-containing processes extended from cortical and medullary epithelial cells, closely contacting thymocytes. Central memory CD8 T cells, which require IL-7 and home to bone marrow, physically associated with IL-7-producing cells as we demonstrate by intravital imaging.

PMID:
19907640
PMCID:
PMC2770321
DOI:
10.1371/journal.pone.0007637
[Indexed for MEDLINE]
Free PMC Article

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