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Dalton Trans. 2009 Dec 7;(45):9970-8. doi: 10.1039/b913426n. Epub 2009 Oct 27.

Recombinant and in vitro expression systems for hydrogenases: new frontiers in basic and applied studies for biological and synthetic H2 production.

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Biosciences Center, National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO, USA.

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  • Dalton Trans. 2010 Dec 28;39(48):11688.


This review focuses on recent progress in developing heterologous and recombinant expression as well as in vitro maturation systems for the biosynthesis of active [FeFe] and [NiFe]-hydrogenases, which catalyze the reversible reaction, H2 <--> 2e- + 2H+. Activities of [FeFe] and [NiFe]-hydrogenases produced from different recombinant and in vitro maturation approaches are compared. Examples of how hydrogenase expression supports basic and applied studies of these enzymes are presented, and barriers to achieving more viable biological and synthetic H2-production systems and catalysts are addressed.

[Indexed for MEDLINE]

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