Format

Send to

Choose Destination
Cancer Res. 2009 Dec 1;69(23):9090-5. doi: 10.1158/0008-5472.CAN-09-2552. Epub 2009 Nov 10.

MiR-15a and MiR-16 control Bmi-1 expression in ovarian cancer.

Author information

1
Department of Biochemistry, College of Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA. bhattacharya.resham@mayo.edu

Abstract

Oncogenic activation of Bmi-1 is found in a wide variety of epithelial malignancies including ovarian cancer, yet a specific mechanism for overexpression of Bmi-1 has not been determined. Thus, realizing the immense pathologic significance of Bmi-1 in cancer, we wanted to investigate if microRNA (miRNA) aberrations played a role in the regulation of Bmi-1 in ovarian cancer. In this report, we identify two miRNAs, miR-15a and miR-16, that are underexpressed in ovarian cell lines and in primary ovarian tissues. We show that these miRNAs directly target the Bmi-1 3' untranslated region and significantly correlate with Bmi-1 protein levels in ovarian cancer patients and cell lines. Furthermore, Bmi-1 protein levels are downregulated in response to miR-15a or miR-16 expression and lead to significant reduction in ovarian cancer cell proliferation and clonal growth. These findings suggest the development of therapeutic strategies by restoring miR-15a and miR-16 expression in ovarian cancer and in other cancers that involve upregulation of Bmi-1.

PMID:
19903841
PMCID:
PMC2859686
DOI:
10.1158/0008-5472.CAN-09-2552
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center