Clonally expanded plasma cells in the cerebrospinal fluid of patients with central nervous system autoimmune demyelination produce "oligoclonal bands"

J Neuroimmunol. 2010 Jan 25;218(1-2):134-9. doi: 10.1016/j.jneuroim.2009.10.005. Epub 2009 Nov 8.

Abstract

Clonally expanded plasma cells (cePC) and oligoclonal IgG (oligoclonal bands, OCB) in the cerebrospinal fluid (CSF) suggest an involvement of B cell mechanisms in autoimmune CNS demyelination. Due to their CSF-restricted occurrence, OCB are commonly believed to be the products of B cells inside the borders of the blood brain barrier. A comparison of CSF cell Ig transcriptomes and CSF-Ig proteomes recently demonstrated, that in multiple sclerosis patients CSF cells are the origin of CSF immunoglobulins. We expand these findings by applying anti-idiotypic antibodies to detect specific heavy chain CDR3 idiotopes of cePC-produced antibodies amongst OCB in the CSF of a patient each with MS and acute disseminated encephalomyelitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Anti-Idiotypic
  • Demyelinating Autoimmune Diseases, CNS / cerebrospinal fluid*
  • Demyelinating Autoimmune Diseases, CNS / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Male
  • Oligoclonal Bands / cerebrospinal fluid*
  • Plasma Cells / immunology*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Antibodies, Anti-Idiotypic
  • Oligoclonal Bands