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Proteomics. 2010 Jan;10(1):164-7. doi: 10.1002/pmic.200900570.

The effect of interfering ions on search algorithm performance for electron-transfer dissociation data.

Author information

1
Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.

Abstract

Collision-activated dissociation and electron-transfer dissociation (ETD) each produce spectra containing unique features. Though several database search algorithms (e.g. SEQUEST, MASCOT, and Open Mass Spectrometry Search Algorithm) have been modified to search ETD data, this consists chiefly of the ability to search for c- and z(*)-ions; additional ETD-specific features are often unaccounted for and may hinder identification. Removal of these features via spectral processing increased total search sensitivity by approximately 20% for both human and yeast data sets; unique peptide identifications increased by approximately 17% for the yeast data sets and approximately 16% for the human data set.

PMID:
19899080
PMCID:
PMC2801774
DOI:
10.1002/pmic.200900570
[Indexed for MEDLINE]
Free PMC Article

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