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Biosci Biotechnol Biochem. 2009 Nov;73(11):2556-9. Epub 2009 Nov 7.

Development of Gateway binary vectors, R4L1pGWBs, for promoter analysis in higher plants.

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Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, Matsue, Japan.


We developed a new series of Gateway binary vectors for plant transformation, R4L1pGWBs, which allow easy construction of promoter:reporter clones. R4L1pGWBs contain a recombination attR4-attL1-reporter cassette, and thus an attL4-promoter-attR1 entry clone was efficiently incorporated by the Gateway LR reaction, resulting in the generation of an attB4-promoter-attB1-reporter construct. The reporters employed in R4L1pGWBs were beta-glucuronidase (GUS), luciferase (LUC), enhanced yellow fluorescent protein (EYFP), enhanced cyan fluorescent protein (ECFP), G3 green fluorescent protein (G3GFP), G3GFP-GUS, and tag red fluorescent protein (TagRFP).

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