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Cell Stem Cell. 2009 Nov 6;5(5):515-26. doi: 10.1016/j.stem.2009.08.017.

High levels of Id1 expression define B1 type adult neural stem cells.

Author information

1
Sloan-Kettering Memorial Cancer Center, Sloan-Kettering Institute, New York, NY 10065, USA. hyn2001@med.cornell.edu

Abstract

Defining the molecular identity of stem cells may be critical for formulating a rational strategy for the therapeutic intervention of stem cell dysfunction. We find that high expression of Id1, a dominant-negative helix-loop-helix transcriptional regulator, identifies a rare population of GFAP(+) astrocytes with stem cell attributes among the subventricular astrocytes in the adult brain. The rare, long-lived, and relatively quiescent Id1(high) astrocytes with morphology characteristic of B1 type astrocytes self-renew and generate migratory neuroblasts that differentiate into olfactory bulb interneurons. Cultured Id1(high) neural stem cells can self-renew asymmetrically and generate a stem and a differentiated cell expressing progressively lower levels of Id1, revealing an Id1 gradient in unperturbed cells of subventricular neurogenic lineages. Moreover, Id genes are necessary to confer self-renewal capacity, a characteristic of stem cell identity. We suggest that high expression of a single transcriptional regulator, Id1, molecularly defines the long-sought-after B1 type adult neural stem cells.

PMID:
19896442
PMCID:
PMC2775820
DOI:
10.1016/j.stem.2009.08.017
[Indexed for MEDLINE]
Free PMC Article

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