Format

Send to

Choose Destination
See comment in PubMed Commons below
Cancer Lett. 2010 Apr 28;290(2):192-203. doi: 10.1016/j.canlet.2009.09.010. Epub 2009 Nov 5.

Selective death of human breast cancer cells by lytic immunoliposomes: Correlation with their HER2 expression level.

Author information

  • 1Molecular and Cellular Biology Institute, Miguel Hernández University, Alicante, Spain.

Abstract

Trastuzumab (Herceptin) targets the human epidermal growth factor receptor 2 (HER2), which is overexpressed in 20-30% of breast and ovarian cancers carrying a bad prognosis. Our purpose was to target HER2-overexpressing human breast cancer cells with pegylated immunoliposomes bearing trastuzumab and containing melittin, which has recently shown anticancer properties. Using a panel of human breast cancer cells with different HER2 expression levels, these immunoliposomes decreased cancer cells viability in a dose-response manner and in correlation to their level of HER2 expression. Specific binding of the immunoliposomes to SKBr3 breast cancer cells was shown by ImageStream-based analysis. The morphological changes observed in the treated cells suggested a cytolytic process. This preclinical approach may suppose an effective strategy for the treatment of HER2-overexpressing tumors, and can support the development of an early phases I-II clinical trial. Trastuzumab resistant breast cancer cells (JIMT-1), can also be targeted using this approach.

PMID:
19896266
DOI:
10.1016/j.canlet.2009.09.010
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center