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J Mol Biol. 2010 Jan 29;395(4):686-704. doi: 10.1016/j.jmb.2009.10.063. Epub 2009 Nov 3.

Molecular evolution of multisubunit RNA polymerases: structural analysis.

Author information

1
The Rockefeller University, Box 224, 1230 York Avenue, New York, NY 10065, USA.

Abstract

Comprehensive multiple sequence alignments of the multisubunit DNA-dependent RNA polymerase (RNAP) large subunits, including the bacterial beta and beta' subunits and their homologs from archaebacterial RNAPs, eukaryotic RNAPs I-III, nuclear-cytoplasmic large double-stranded DNA virus RNAPs, and plant plastid RNAPs, were created [Lane, W. J. and Darst, S. A. (2009). Molecular evolution of multisubunit RNA polymerases: sequence analysis. In press]. The alignments were used to delineate sequence regions shared among all classes of multisubunit RNAPs, defining common, fundamental RNAP features as well as identifying highly conserved positions. Here, we present a systematic, detailed structural analysis of these shared regions and highly conserved positions in terms of the RNAP structure, as well as the RNAP structure/function relationship, when known.

PMID:
19895816
PMCID:
PMC2813324
DOI:
10.1016/j.jmb.2009.10.063
[Indexed for MEDLINE]
Free PMC Article

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